PCNA (aa111-122) Antibody
Goat polyclonal to PCNA.Reported variants represent identical protein: NP_872590.1, NP_002583.1 .
Product Specifications
Product Name Alternative
Anti cyclin antibody, DNA polymerase delta auxiliary protein antibody, MGC8367 antibody, OTTHUMP00000030189 antibody, OTTHUMP00000030190 antibody, PCNA antibody, proliferating cell nuclear antigen antibody
Reactivity
Human, Mouse, Porcine, Rat
Target
PCNA (aa111-122)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EAPNQEKVSDYE
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
Molecular Weight
28.8
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1) . Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates. (calcu. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
5111
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