What is the catalog number for PCNA (aa111-122) Antibody?

The catalog number for PCNA (aa111-122) Antibody is orb43327.

What sizes are available for PCNA (aa111-122) Antibody?

PCNA (aa111-122) Antibody is available in the following size(s): 100 µg.

Is PCNA (aa111-122) Antibody in stock?

PCNA (aa111-122) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

PCNA (aa111-122) Antibody

Goat polyclonal to PCNA.Reported variants represent identical protein: NP_872590.1, NP_002583.1 .

Product Specifications

Product Name Alternative

Anti cyclin antibody, DNA polymerase delta auxiliary protein antibody, MGC8367 antibody, OTTHUMP00000030189 antibody, OTTHUMP00000030190 antibody, PCNA antibody, proliferating cell nuclear antigen antibody

Reactivity

Human, Mouse, Porcine, Rat

Target

PCNA (aa111-122)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EAPNQEKVSDYE

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

28.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1) . Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates. (calcu. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.