Recombinant Human CDH1 Protein (His Tag)

Product Specifications

Background

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Abbreviation

CDH1

Synonyms

CDH; Arc; CADH; CD antigen CD; Cleaved into: E-Cad/CTF; Cadherin; CAM 120/; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; CDH1; E-Cadherin; CADH1; Cadherin-1; Uvomorulin; E-Cad; CTF2; CAM 120/80; Epithelial cadherin; CD antigen CD324; Cleaved into: E-Cad/CTF1; CTF3; CDH1; Arc 1; Arc 1; CADH1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324; CD324 antigen; cdh1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; Arc-1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CD 324; CD324 antigen; E-Cad/CTF3; epithelial calcium dependant adhesion protein; Liver cell adhesion molecule

UniProt

P12830

Accession Number

P12830

Expression System

Mammalian

Tag

C-His

Sequence

Asp155-Ala709

Field of Research

Cancer; Cardiovascular; Cell Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.