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NOTCH4 (aa1261-1273) Antibody

Goat polyclonal antibody to NOTCH4

Product Specifications

Product Name Alternative

Anti NOTCH4 antibody, anti DAQB-143M3.4 antibody, anti FLJ16302 antibody, anti INT3 antibody, anti MGC74442 antibody, anti NOTCH3 antibody, anti Notch homolog 4 antibody, anti neurogenic locus notch homolog protein 4 antibody

Reactivity

Human, Mouse

Target

NOTCH4 (aa1261-1273)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DHFHNGHCEKGCNN

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 1 μg/ml, IHC-P: 10 μg/ml

Molecular Weight

210

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Preliminary experiments gave an approx 27kDa band in all human, mouse and rat tissue and cell line lysates tested after 1μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10μg/ml.

Prediction Reactivity

Bovine, Canine, Porcine, Rat

Tested Applications

ELISA, IF

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

4855

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