NOX5 Antibody

Goat polyclonal antibody to NOX5

Product Specifications

Product Name Alternative

Anti NOX5 antibody, anti NADPH oxidase, EF-hand calcium binding domain 5 antibody, anti MGC149776 antibody, anti MGC149777 antibody, anti NADPH oxidase 5 antibody, anti OTTHUMP00000164443 antibody, anti OTTHUMP00000164444 antibody

Reactivity

Human

Target

NOX5

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EWHPFTISSAPEQKD

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 10ug/ml

Molecular Weight

86.4; 83.8; 83.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.4kDa according to NP_078781.3) . Recommended concentration: 0.5-2μg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.