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BAPTA

BAPTA is a selective chelator for calcium. BAPTA, as calcium indicator, has high selectivity against magnesium and calcium. BAPTA is widely used as an intracellular buffer for investigating the effects of Ca2+ release from intracellular stores or influx via Ca2+-permeable channels in the plasma membrane. BAPTA can also inhibit phospholipase C activity independently of their role as Ca2+ chelators[1][2].

Product Specifications

CAS Number

[85233-19-8]

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Phospholipase

Type

Biochemical Assay Reagents

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/BAPTA.html

Concentration

10mM

Purity

98.10

Solubility

1M NaOH : 33.33 mg/mL (ultrasonic; adjust pH to 11 with NaOH) |DMSO : 15.62 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C(O)CN(C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(O)=O)CC(O)=O)CC(O)=O

Molecular Formula

C22H24N2O10

Molecular Weight

476.43

Precautions

H302, H315, H319, H335

References & Citations

[1]R Y Tsien, et al. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry. 1980 May 27;19 (11) :2396-404. |[2]Roger C Hardie, et al. Inhibition of phospholipase C activity in Drosophila photoreceptors by 1,2-bis (2-aminophenoxy) ethane N, N, N', N'-tetraacetic acid (BAPTA) and di-bromo BAPTA. Cell Calcium. 2005 Dec;38 (6) :547-56. |[3]M B Collatz, et al. Intracellular calcium chelator BAPTA protects cells against toxic calcium overload but also alters physiological calcium responses. Cell Calcium

Shipping Conditions

Room Temperature

Storage Conditions

-20°C, 3 years; 4°C, 2 years (Powder)

Scientific Category

Biochemical Assay Reagents

Clinical Information

No Development Reported

Citation 01

Adv Sci (Weinh) . 2021 Aug;8 (15) :e2100363.|Aging. 2019 Nov 22;11 (22) :10385-10408.|Biochim Biophys Acta Mol Basis Dis. 2025 Jul 18;1871 (8) :167990.|Biomed Pharmacother. 2023 May:161:114480.|Biomed Pharmacother. 2023 Oct:166:115343.|bioRxiv. 2023 Jun 25.|Cardiovasc Ther. 2023 Mar 30:2023:3939360.|Environ Chem Ecotoxicol. 2025 Nov 19;8:267-280.|Exp Neurol. 2023 May:363:114350.|Free Radic Biol Med. 2024 Mar:213:150-163.|Int J Pharm. 2025 Nov 10:684:126125.|J Cancer. 2024 Jan 1;15 (2) :343-355.|J Cell Mol Med. 2020 Aug;24 (16) :9287-9299.|J Clin Invest. 2024 Apr 25;134 (12) :e176355.|J Ethnopharmacol. 2023 Feb 10;302 (Pt A) :115923.|J Ethnopharmacol. 2025 Apr 29:348:119863.|J Funct Foods. 2024 Oct.|J Funct Foods. December 2021, 104791.|J Ginseng Res. 2022 Jul;46 (4) :515-525.|J Invest Dermatol. 2024 May 30:S0022-202X (24) 00384-1.|J Neuroinflammation. 2025 Aug 28;22 (1) :208.|J Pharm Pharmacol. 2024 May 3;76 (5) :567-578.|J Pineal Res. 2024 Aug;76 (5) :e12987.|J Thromb Haemost. 2021 Dec;19 (12) :3113-3125.|Mil Med Res. 2023 Nov 25;10 (1) :56.|Mol Med Rep. 2024 Jan;29 (1) :13.|Res Sq. 2025 Apr 18.|Sci Immunol. 2019 Jun 28;4 (36) :eaau6426.|Sci Total Environ. 2020 Feb 10;703:134702.|Toxicology. 2023 Dec:500:153674.|Vet Microbiol. 2019 Dec;239:108498.|Cell Signal. 2025 Jul:131:111753.

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