IGF1 Antibody

Goat Polyclonal to IGF1. NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1, PMID: 20230795 and GeneID: 3479.

Product Specifications

Product Name Alternative

Anti IGF1 antibody, anti insulin-like growth factor 1 (somatomedin C) antibody, anti IGF1A antibody, anti IGFI antibody, anti OTTHUMP00000195084 antibody, anti insulin-like growth factor 1 antibody

Reactivity

Human

Target

IGF1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RSVRAQRHTD

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

17.8; 15.2; 21.8; 17.0

References & Citations

Wang, Yan et al. Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows Animals (Basel), 10, E357 (2020)

Pubmed id

32102173

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1) . Recommended concentration 0.5-2μg/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Prelimi. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.