Nitric Oxide Synthase Assay
This kit utilizes a cell-permeant dye, diaminofluorescein-2 Diacetate (DAF-2DA) to measure nitric oxide and nitric oxide synthase activity in living cells and tissue sections. Read using a fluorescence plate reader or microscope.
Product Specifications
Label
ICT
Type
Cell Viability
Detection Method
Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy
Assay Protocol
https://www.celltechnology.com/wp-content/uploads/2020/12/NOSProtocol.pdf
Components
Part# 4006: DAF-2DA, 1 Vial (NOS200-1) (2-8C)
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Calculated Molecular Weight
446.4
Target Description
Diaminofluorescein-2 Diacetate (DAF-2DA) is a non-fluorescent cell permeable reagent that can measure free Nitric Oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the diacetate groups on the DAF- 2DA reagent are hydrolyze by cytosolic esterases thus releasing DAF-2 and sequestering the reagent inside the cell. Production of nitric oxide converts the non-fluorescent dye, DAF-2, to its fluorescent triaole derivative, DAF-2T. DAF-2T can be observed by: excitation 488 nm and measuring emission at 515 nm.
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
