What is the catalog number for Nitric Oxide Synthase Assay?

The catalog number for Nitric Oxide Synthase Assay is NOS200-1.

Is Nitric Oxide Synthase Assay in stock?

Nitric Oxide Synthase Assay is currently made to order / not held in immediate stock. Contact us for lead time.

Nitric Oxide Synthase Assay

This kit utilizes a cell-permeant dye, diaminofluorescein-2 Diacetate (DAF-2DA) to measure nitric oxide and nitric oxide synthase activity in living cells and tissue sections. Read using a fluorescence plate reader or microscope.

Product Specifications

Label

ICT

Type

Cell Viability

Detection Method

Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy

Assay Protocol

https://www.celltechnology.com/wp-content/uploads/2020/12/NOSProtocol.pdf

Components

Part# 4006: DAF-2DA, 1 Vial (NOS200-1) (2-8C)

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Calculated Molecular Weight

446.4

Target Description

Diaminofluorescein-2 Diacetate (DAF-2DA) is a non-fluorescent cell permeable reagent that can measure free Nitric Oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the diacetate groups on the DAF- 2DA reagent are hydrolyze by cytosolic esterases thus releasing DAF-2 and sequestering the reagent inside the cell. Production of nitric oxide converts the non-fluorescent dye, DAF-2, to its fluorescent triaole derivative, DAF-2T. DAF-2T can be observed by: excitation 488 nm and measuring emission at 515 nm.