Fluorescent Cell Proliferation Assay

Measurement of cell proliferation and viability is frequently used in clinical and experimental immunology as means of assessing cell activation in response to diseases, infections and environmental stimulations. This assay can be used for the measurement of cell proliferation in response to antigens, cytokines, growth factors, and mitogens. It can also be used for the analysis of cytotoxic effects of anticancer drugs, drug resistance, cytotoxic pharmaceutical compounds, and other toxic agents. This kit uses an oxidation/reduction-based reagent that functions as a cell viability indicator by quantitatively measuring the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When added to cells, the reagent is modified by the reducing environment of the viable cell, it turns red in color and becomes highly fluorescent. This change can be detected using fluorescence or absorbance measurements. The reagent is detected at Excitation = 530 nm and Emission = 590 nm. Absorbance is measured at 570 nm. Compared to the traditional cell proliferation assay, our novel in-cell-culture method is simple, fast and sensitive. The entire assay can be performed in a 96-Well microtiter plate in 30 minutes and the best results are achieved in 3 hours.