Hydrogen Peroxide Detection/Peroxidase Detection Kit

Part # 3011: 5X Reaction Buffer: 20 ml buffer, pH 7.4 (2-8°C), Part # 4007: Detection reagent: One vial for 500 assays. (2-8°C), Part # 3012: Hydrogen Peroxide: 200µL of a stabilized 3% solution. (2-8°C), Part # 6004: Horseradish Peroxidase: 18.9 Units of enzyme (2-8°C)

The kit utilizes a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent to produce a fluorescent product, resorufin which is catalyzed by peroxidase in a homogeneous no wash assay system. The detection reagent can be utilized to measure H2O2 release from cells or enzyme coupled reactions.