Fluorescent Lactate Detection Kit

Part # 7022: Lactate Standard 4mM, 500µl (2-8C), Part # 6004: Horseradish Peroxidase, 18.9 Units (2-8C), Part # 3011: 5X Reaction Buffer, 25 mL (2-8C), Part # 4026: Detection Reagent, 1 Vial (-20C), Part # 6025: Reaction Enzyme Mix, 1 Vial - 1.1mL (-20C)

Lactate is an intermediate product of carbohydrate metabolism. Of the two forms of Lactate, D- and L-, the L-lactate is the predominant isomer found in biological systems. L-lactate is formed during the anaerobic glycolysis by conversion of pyruvate to L-lactate by lactate dehydrogenase. Lactate level is an indicator for tissue oxygen demand and utilization. Abnormally high lactate levels are associated with diseases such as diabetes and lactate acidosis. Our lactate assay is a lactate oxidase-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and tissue extracts. In the assay, lactate oxidase (LOX) catalyzes the oxidation of L-lactate to pyruvate, along with the concomitant reduction of hydrogen peroxide (H2O2). The detection utilizes a non-fluorescent detection reagent, which is oxidized in the presence of horseradish peroxidase and LOX to produce its fluorescent analog.