Fluorescent ATP Detection Kit

ICT’s Fluoro ATP assay provides a reliable, sensitive fluorimetric assay for the quantification of ATP in biological samples., Detection of ATP in cells or tissue extracts., Detection of ATP in cell death, energy metabolism, mitochondria function., ATP measurement in ATP consuming enzymes such as Kinases and ATPases., ATP detection in Bacterial, Fungal and Plant Cells.

Product Specifications

Label

ICT

Type

Metabolic Assays

Detection Method

Fluorescence plate reader

Wavelength

530-570nm/590-600nm

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_ATP_Protocol.pdf?v=1693430763

Components

1. Part #: 6032. Enzyme Mix 25X: 5 frozen vials 220 μL. Upon arrival store at -20 °C., Aliquot and store at -20 °C., 2. Part# 3065: Substrate Buffer. 5 bottle 25mL. Upon arrival store at -20 °C. Ready to use., 3. Part# 4028: Detection Reagent 100X: 5 dried vials. Upon arrival store -20 °C, To reconstitute add 60 μL of anhydrous DMSO. Aliquot and store at -20 °C, 4. Part# 7027: ATP Standard 2.5mM: 5 vials. Upon arrival store -20 °C, After first use aliquot and store at -20 °C, 5. Part# 7026: NEM: 5 vials dried. Reconstitute in 0.5mL of ETOH. Upon arrival and reconstitution store -20 °C. Dilute as needed.

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

-20°C

Target Description

The Fluoro ATP detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence ATP and a coupled enzyme reaction to produce its fluorescent analog. There is a linear relationship of ATP concentration to the fluorescent analog concentration. An ATP standard curve is generated to interpolate sample ATP concentrations. The kit can be used in both endpoint and kenitic modes.