Fluorescent ATP Detection Kit

1. Part #: 6032. Enzyme Mix 25X: 5 frozen vials 220 μL. Upon arrival store at -20 °C., Aliquot and store at -20 °C., 2. Part# 3065: Substrate Buffer. 5 bottle 25mL. Upon arrival store at -20 °C. Ready to use., 3. Part# 4028: Detection Reagent 100X: 5 dried vials. Upon arrival store -20 °C, To reconstitute add 60 μL of anhydrous DMSO. Aliquot and store at -20 °C, 4. Part# 7027: ATP Standard 2.5mM: 5 vials. Upon arrival store -20 °C, After first use aliquot and store at -20 °C, 5. Part# 7026: NEM: 5 vials dried. Reconstitute in 0.5mL of ETOH. Upon arrival and reconstitution store -20 °C. Dilute as needed.

The Fluoro ATP detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence ATP and a coupled enzyme reaction to produce its fluorescent analog. There is a linear relationship of ATP concentration to the fluorescent analog concentration. An ATP standard curve is generated to interpolate sample ATP concentrations. The kit can be used in both endpoint and kenitic modes.