Fluorescent ATP Detection Kit
ICT’s Fluoro ATP assay provides a reliable, sensitive fluorimetric assay for the quantification of ATP in biological samples., Detection of ATP in cells or tissue extracts., Detection of ATP in cell death, energy metabolism, mitochondria function., ATP measurement in ATP consuming enzymes such as Kinases and ATPases., ATP detection in Bacterial, Fungal and Plant Cells.
Product Specifications
Label
ICT
Type
Metabolic Assays
Detection Method
Fluorescence plate reader
Wavelength
530-570nm/590-600nm
Assay Protocol
https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Fluoro_ATP_Protocol.pdf?v=1693430763
Components
1. Part #: 6032. Enzyme Mix 25X: 5 frozen vials 220 μL. Upon arrival store at -20 °C., Aliquot and store at -20 °C., 2. Part# 3065: Substrate Buffer. 5 bottle 25mL. Upon arrival store at -20 °C. Ready to use., 3. Part# 4028: Detection Reagent 100X: 5 dried vials. Upon arrival store -20 °C, To reconstitute add 60 μL of anhydrous DMSO. Aliquot and store at -20 °C, 4. Part# 7027: ATP Standard 2.5mM: 5 vials. Upon arrival store -20 °C, After first use aliquot and store at -20 °C, 5. Part# 7026: NEM: 5 vials dried. Reconstitute in 0.5mL of ETOH. Upon arrival and reconstitution store -20 °C. Dilute as needed.
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
-20°C
Target Description
The Fluoro ATP detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence ATP and a coupled enzyme reaction to produce its fluorescent analog. There is a linear relationship of ATP concentration to the fluorescent analog concentration. An ATP standard curve is generated to interpolate sample ATP concentrations. The kit can be used in both endpoint and kenitic modes.
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