Fluorescent ADP Detection Kit

1. Part #: 6031. Enzyme Mix 25X: 1 frozen vial 220 μL. Upon arrival store at -20 °C., Aliquot and store at -20 °C., 2. Part# 3064: Substrate Buffer. 1 bottle 25mL. Upon arrival store at -20 °C. Ready to use., 3. Part# 4028: Detection Reagent 100X: 1 dried vial. Upon arrival store -20 °C, To reconstitute add 60 μL of anhydrous DMSO. Aliquot and store at -20 °C, 4. Part# 7025: ADP Standard 2.5mM: 1 vial. Upon arrival store -20 °C, After first use aliquot and store at -20 °C, 5. Part# 7026: NEM: 1 vial dried. Reconstitute in 0.5mL of ETOH. Upon arrival and reconstitution store -, 20C0. Dilute as needed

Adenosine diphosphate (ADP) is an organic molecule which plays a central role in cellular metabolism and energy transfer reactions. ADP is a product of ATP de-phosphorylation and can be rephosphorylated to ATP or further de-phosphorylation to AMP. This cycling of ADP is central to transferring potential (thermodynamic) energy from one source to another. Besides its involvement in glycolysis, citric acid cycle and oxidative phosphorylation, ADP is also stored as dense bodies in blood platelets. Upon platelet activation, ADP is release which leads to further platelet activation through ADP receptors. Fluorescent ADP Detection Kit utilizes a non-fluorescent detection reagent, which is reduced in the presence of ADP and a coupled enzyme reaction to produce its fluorescent analog. There is a linear relationship of ADP concentration to the fluorescent analog concentration. An ADP standard curve is generated to interpolate sample ADP concentrations. The kit can be used in both endpoint and kinetic modes. The kit can be used with mammalian cells and tissues, and bacterial, fungal and plant cells.