Plasma Sample Diluent (Antigen-Down SD)

Simply dilute the buffer 1:10 (100mL WB to 900mL diH20) and it’s ready to use. To rinse microtiter plates, the buffer may be dispensed through a squirt bottle, a plate washer, or a multi-channel pipette. Be sure to fill the Wells all the way up (400uL for a 96-Well plate). We do NOT recommend submerging the entire plates in a bath as many of the Wells become contaminated, which decreases the reliability of the assay.

Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Additional additives have been included to inhibit thrombin (clotting) and complement activity during the plasma sample incubation period. ESD200 also contains an antimicrobial agent to provide a solution free of bacterial contamination.