Plasma Sample Diluent (Antigen-Down SD)

Simply multiply the value determined from the curve by your dilution factor. For example, if you diluted your sample 1:2 (250uL sample into 250uL sample diluent) and this yielded an OD of 0.437, when read off the curve, the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL, meaning that the original starting sample had 400pg/mL of analyte present.

Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Additional additives have been included to inhibit thrombin (clotting) and complement activity during the plasma sample incubation period. ESD200 also contains an antimicrobial agent to provide a solution free of bacterial contamination.