Nonmammalian-Based Blocker

Once your plate has been coated with an antibody or antigen, aspirate or dump out the coating solution. Then, simply pipette 300-400uL per Well of block buffer and let it incubate (Cell Technology recommends a minimum incubation of 3 hours).

ICT’s Nonmammalian-Based Blocker contains a proprietary non-mammalian formulation based on fish proteins, plus molecular stabilizers that provide a high degree of blocking efficiency. EBB200 was designed for antigen-down ELISA formats, and for sandwich assays with high background problems. This formulation is a heterogeneous mixture of small molecules capable of blocking both the nonspecific binding sites of the absorbed protein, as Well as the unoccupied regions of the polystyrene plate that are not sterically accessible to larger traditional mammalian blocking reagents, thereby reducing background noise. The small size of these unique blockers results in minimal steric hindrance to key epitope regions of target coated proteins, thus enhancing specific signal generation., EBB200 reduces the possibility of false-positives generated from endogenous antibodies in the sample reacting with plate blocking proteins. It is particularly useful when working with human, swine, and bovine serum samples, as there is little interaction with the blocking molecules resulting in lower backgrounds.