aCella - Tox - an assay designed to measure cell cytoxicity

1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C), 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C), 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C), 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C), 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C), 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C),

GAPDH is an important enzyme in the glycolysis and gluconeogenesis pathways. This homotetrameric enzyme catalyzes the oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate in the presence of cofactor and inorganic phosphate. In the aCella-TOX reaction scheme the release of GAPDH is coupled to the activity of the enzyme 3-Phosphoglyceric Phosphokinase (PGK) to produce ATP. ATP is detected via the luciferase, luciferin Bioluminescence methodology. Further, aCella-TOX is a homogeneous cytotoxicity assay; alternatively in dual mode, aCella-TOX can measure cytotoxicity and cell viability in the same plate. Culture supernatants can also be removed from the original plate and assayed in a different plate, allowing kinetics runs to be set up. The assay is non-destructive, allowing the monitoring of additional parameters such as gene expression.