Peroxidase, Unconjugated - Bader says not IHC Reagent?
Horseradish peroxidase (HRP) is a glycoprotein, MW approximately 44 kDa, with hemin prosthetic group and bound Ca++. Peroxidase oxidizes many chromogenic substrates (ABTS, TMB, o-dianiside, 5-Aminosalicylic acid (5AS), DAB) to form color complexes; chemiluminescent (luminal and isoluminol) and fluorogenic (tyramine, homovanillic acid and 4-hydroxyphenyl acetic acid). These are used in ELISA, immunoblots, and Immunohistochemistry.
Product Specifications
Other Statements
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
Label
ICT
Type
IHC Reagents
Applications
IHC
Assay Protocol
The product is supplied as salt free in distilled water. No preservative have been added. On arrival aliquot and freeze at -20oC. Proclin 300 (final concentration 0.05%) can be added as a preservative and then the enzyme can be stored at 2-8oC.
DO NOT USE Sodium azide as preservative in buffers or washing steps as it will inhibit the enzyme activity.
Buffer
6.0-6.5
pH
6.0-6.5
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
-20°C
Target Description
Horseradish peroxidase (HRP) is a glycoprotein, MW approximately 44 kDa, with hemin prosthetic group and bound Ca++. Peroxidase oxidizes many chromogenic substrates (ABTS, TMB, o-dianiside, 5-Aminosalicylic acid (5AS), DAB) to form color complexes; chemiluminescent (luminal and isoluminol) and fluorogenic (tyramine, homovanillic acid and 4-hydroxyphenyl acetic acid). These are used in ELISA, immunoblots, and Immunohistochemistry.
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