Immuno Automation Buffer™ 10X

This buffer is supplied as 10X solution. It is used as 1X in all immunoassays (Immunofluorescence, IHC, ELISA, WB etc), as a washing buffer and can be used as dilution buffer for antibodies; however it does not contain any carrier proteins. This buffer does not contain any phosphate, sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase, and antibodies to phosphoproteins. Not suitable for dilution of antibodies to S100 proteins. This buffer does not contain any proteins.

Preparation: Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8oC for several weeks. Application: Wash 3-5X with this buffer in between steps in all immunoassays protocol.

This buffer is supplied as 10X solution. It is used as 1X in all immunoassays (Immunofluorescence, IHC, ELISA, WB etc), as a washing buffer and can be used as dilution buffer for antibodies; however it does not contain any carrier proteins. This buffer does not contain any phosphate, sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase, and antibodies to phosphoproteins. Not suitable for dilution of antibodies to S100 proteins. This buffer does not contain any proteins.