HRP Conjugate Stabilizer, Mammalian, 1X

1. Prepare 1X working strength sample diluent by diluting 1:2 in diH2O. Add 1 part General Serum Diluent, 2X to 1 part diH2O. Mix Well., 2. Prepare standards and samples in General Serum Diluent., 3. Generally, serum samples should be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding. Highly concentrated samples may need to be diluted 1:1,000 or more., 4. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 μL sample to 990 μL General Serum Diluent for a total of 1,000 μL., 5. Once diluted, run the assay according to the specific ELISA protocol., 6. Analyze the data. If the samples were diluted, the dilution factors must be considered when calculating their values.

HRP Conjugate Stabilizer, Mammalian, 1X preserves the activity of HRP-protein conjugates during storage, reconstitution, and immunoassay use. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques. HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional antigen-down or antibody sandwich formats. Additional components may be added to HRP Conjugate Stabilizer, Mammalian, 1X to optimize it for particular ELISA format compositions or for use in other immunological techniques. Antimicrobial agents are included to retard bacterial growth., , HRP Conjugate Stabilizer, Mammalian, 1X preserves the functional integrity of the horseradish peroxidase enzyme and immunoglobulin components of stock conjugates upon reconstitution or dilution to its working concentration for ELISA or other immunology-based techniques. Its proprietary formula also stabilizes the HRP conjugate during storage and enhances the bond between detection HRP conjugated antibody and target antigen or antibody., , Mammalian proteins and salt additives provide structural support to both the immunoglobulin and HRP components of the conjugate, preserving enzymatic and IgG activity and stabilizing the conjugate bond., , HRP Conjugate Stabilizer, Mammalian, 1X inhibits the HRP conjugate from binding to nonspecific serum proteins in the sample, thereby reducing conjugate bridging on the ELISA plate surface and reducing non-specific background noise.