Stop Solution for TMB Substrates (STOPT)

1. Protect the HRP conjugate stabilizer from light. This may be done by covering the container with aluminum foil., 2. Add the HRP conjugate at the target concentration to Neptune HRP Conjugate Stabilizer. For example, if diluting the HRP conjugate 1:5000, add 10 µL conjugate stock to 49.99 mL Neptune HRP Conjugate Stabilizer., 3. Mix for 15 minutes., 4. Use the diluted HRP conjugate in the immunoassay., 5. Store remaining HRP conjugate solution at 2-8°C. Protect from direct exposure to light.

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Stop Solution for TMB Substrates (STOPT) is a ready-to-use liquid acidic stop solution suitable for use with our TMB substrates. This product is suitable for all endpoint ELISAs using a TMB substrate for color development., , TMB substrates react with peroxidase, yielding a soluble blue reaction product. When the TMB-peroxidase reaction is stopped using Stop Solution for TMB Substrates, the chromogen changes from blue to yellow, and hence the absorbance from 620 nm to 450 nm., , Bulk volumes are available upon request.