pNPP 1-Component AP MicroWell Substrate with Stabilizing Pellets (SUBP)

1. Allow product to equilibrate to room temperature (25°C) prior to use., 2. Completely cover the membrane surface with the substrate. The substrate will react with peroxidase creating an insoluble permanent dark blue reaction product., 3. To stop the reaction, rinse the developed membrane with high quality water., 4. For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.

pNPP 1-Component AP MicroWell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microWell assays., , Alkaline phosphatase substrates are used in ELISA assays to detect alkaline phosphatase-conjugated molecules via a colorimetric enzymatic reaction. pNPP 1-Component AP MicroWell Substrate with Stabilizing Pellets contains para-Nitrophenylphosphate (pNPP). This formulation of pNPP substrate also contains a stabilizer to extend the shelf life of the alkaline phosphatase substrate on your benchtop to 2-3 days., , The alkaline phosphatase substrate reaction may be stopped with the use of a stop solution, Stop Solution for pNPP AP MicroWell Substrates., , Bulk volumes are available upon request.