Phosph-Free Block ELISA Blocking Buffer

1. Add the antibody-AP or protein-AP conjugate directly into the Alkaline Phosphatase Conjugate Stabilizer to a working 1X dilution and mix for 5-15 minutes., 2. Pipette diluted AP conjugate onto the plate or store protected from light at 2-8°C for use at a later date. 1X working AP conjugate dilutions can be stored at 2-8°C and protected from light providing a useful shelf-life of 6 months.

Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs., , This block buffer is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Because this ELISA buffer contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme labeled conjugates to blocked plate surfaces. Use of this blocking formulation minimizes backgrounds without the use of conventional cross-reactive protein additives., , Phosph-Free Block works on all types of polystyrene plates except Immulon® II microplates. When blocking with this buffer, ICT recommends Corning® 96-Well EIA/RIA StripWell™ microplates (ICT catalog #25).