Antigen-Down HRP Conjugate Stabilizer, 5X

1. Prepare standards and samples in Neptune Sample Diluent., 2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding., 3. To dilute the sample 1:100, add 1 part sample to 99 parts Neptune Sample Diluent. For example, add 10 μL sample to 990 μL sample diluent for a total of 1,000 μL., 4. Highly concentrated samples may need to be diluted 1:1,000 or more., 5. Once diluted, run the assay according to the specific ELISA protocol.

Antigen-Down HRP Conjugate Stabilizer is a unique HRP stabilizer formulation specifically designed for use with anti-IgG-HRP conjugates. This HRP stabilizing diluent contains proprietary non-mammalian protein additives that will not interfere with anti-IgG-HRP conjugates used in antigen-down immunoassays or sandwich ELISA formats. These additives also provide additional structural stability to the IgG-HRP conjugate protein complex., , Antigen-Down HRP Conjugate Stabilizer will also inhibit conjugate binding to nonspecific serum proteins bound to the plate, reducing conjugate bridging on the plate surface and lowering background noise. Antimicrobial agents have been added to retard bacterial growth.