Block Buffer Optimization Pack

1. To quickly determine which Assay Diluent formulation is best for the assay, all four Assay Diluents can be run in parallel on the same IgG-coated ELISA plate., 2. Determine the initial dynamic analyte detection range of the ELISA curves that were prepared in each of the four different Assay Diluent types., 3. Make test spikes into serum samples., 4. Read the spiked serum sample Wells from each of the four AD-associated standard curves to see which Assay Diluent gives the best approximation of the spike concentration that was added to the serum samples., 5. In cases where a high level of matrix complexity interference is not detected, an additional Assay Diluent may not be required to obtain a useful standard curve and derived sample analysis.

After coating the EIA/RIA plate with the capture antigen or antibody, proper blocking of the unoccupied areas of the plate Wells is paramount to attaining an accurate signal. Assay sensitivity improves by utilizing the appropriate blocking buffer that effectively decreases background noise and improves the signal-to-noise ratio. Evaluation of blocking candidates is an important step of ELISA development. The Block Buffer Optimization Pack provides three of ICT’s blocking buffer formulations for an economical and fast method of selecting the best blocking buffer for a particular assay. The Pack includes 100 mL of three ELISA blocking buffers, which is adequate to begin optimizing the blocking stage of the assay development process.