Plasma Sample Diluent

1. Prepare samples and controls, 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 μL DMSO., 4. Dilute FLICA 1:5 by adding 200 μL PBS., 5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 μL to 290 μL of cultured cells)., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells., 8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 9. If desired, fix cells., 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm., , If working with adherent cells, please see the manual for additional protocols.

Plasma Sample Diluent should be utilized as a buffer matrix for diluting out plasma samples in antigen-down or antigen-capture ELISA formats. This diluent is also an excellent choice for diluting out antigen standards in ELISA standard curve preparation. This ELISA sample diluent contains proprietary additives in a buffered BSA protein base providing an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction., , Specially formulated additives inhibit thrombin activity (clotting) and complement activity during the plasma sample incubation period. Plasma Sample Diluent also contains antimicrobial agents to allow for room temperature benchtop use and extensive storage stability., , Bulk volumes and custom packaging are available upon request.