HRP Conjugate Stock Stabilizer, 5X

When diluting test samples, first note the dilution factor for future data analysis. For example, a human plasma sample was diluted 1:10 (50 µL plasma sample + 450 µL plasma sample diluent) and yielded a standard curve derived analyte concentration of 50 ng/mL from the ELISA assay. This concentration should then be multiplied by 10 (the dilution factor) to obtain the actual concentration value of 500 ng/mL in the undiluted plasma sample.

Stabilizes antibody-HRP conjugates while minimizing non-specific signal. HRP Conjugate Stock Stabilizer, 5X is provided as a 5X concentrate. When diluted to its 1X working concentration, it can be used to create concentrated stock conjugates for long-term storage, to reconstitute lyophilized HRP conjugates, and dilute protein-peroxidase conjugates to their useful working titer in ELISAs and other immunology-based techniques. At 1X, HRP Conjugate Stock Stabilizer maintains the functional integrity of the horseradish peroxidase enzyme and immunoglobulin components of the HRP-IgG conjugate complex. This proprietary formulation helps preserve the native three-dimensional conjugate structure during storage while concurrently enhancing the steric interaction or binding relationship between the IgG component of the HRP- IgG conjugate and the target antigen or antibody during the assay. By maintaining conjugate component activity and preventing contamination issues through the inclusion of an anti-microbial agent, HRP Conjugate Stock Stabilizer extends the functional utility of stored and reconstituted peroxidase conjugates. In addition, it inhibits the conjugate from non-specifically binding to plate coating proteins or non-target sample proteins. By reducing non-specific conjugate bridging events on the plate surface, undesirable background signal-related losses in sensitivity can be all but eliminated. Additional components may be added to HRP Conjugate Stock Stabilizer to optimize it for unique ELISA sample situations or for use in other immunological techniques.