ELISA Wash Buffer, 10X

When using HRP Conjugate Stock Stabilizer, 5X as a Conjugate Diluent:, 1. Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes., 2. Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes., 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C, protected from light, providing a useful shelf-life of 6-12 months., , When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer:, 1. To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer., 2. Filter sterilize into a sterile amber or brown-glass container., 3. Store at 2-8°C. Concentrated HRP conjugate stocks should be stable for up to 18 months.

An optimal formulation of salts and detergents to wash ELISA plates. ELISA Wash Buffer, 10X is used to remove unbound components from microtiter plates after the coating process and between reagent addition steps of an ELISA. ICT’s ELISA Wash Buffer is an optimal formulation of pH stabilizers, salts, and detergents designed to effectively remove excess material from the microtiter plate Wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound assay components and interfering substances can be washed away without suppressing antigen-antibody binding interactions, thereby reducing non-specific background noise and increasing the specific signal. ICT’s ELISA Wash Buffer, 10X is a universal ELISA wash buffer; it may be used with antibody-sandwich ELISAs and antigen-down ELISAs. It contains a non-azide, non-mercury preservative that will not interfere with the assay binding interactions, while still providing excellent long-term, 1X storage stability at room temperature. As it is supplied concentrated at 10X, crystalline precipitates may form in the bottle, especially when refrigerated. If this occurs, gently warm or mix the buffer until all crystals have dissolved.