Antibody Coating Buffer, 5X

1. Prepare standards and samples in General Serum Diluent., 2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding., 3. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 μL sample to 990 μL sample diluent for a total of 1,000 μL., 4. Highly concentrated samples may need to be diluted 1:1,000 or more., 5. Once diluted, run the assay according to the specific ELISA protocol.

Enhances adsorption of antibodies while preserving structure. Antibody Coating Buffer, 5X is a protein-stabilizing solution that maximizes the adsorption of capture antibodies onto polystyrene plates. During the plate coating process, the salt and pH buffering environment provided by Antibody Coating Buffer stabilizes the three-dimensional antibody structure, preserving the antigen recognition regions of the antibody. This buffered environment also provides a highly consistent adsorption rate across all Wells of the ELISA plate. In addition, this unique protein stabilization buffer may allow for the use of lower quantities of valuable capture antibody. Therefore, the use of Antibody Coating Buffer allows ELISA plates to be manufactured with high levels of precision and antigen capture utility. As Antibody Coating Buffer is concentrated 5X, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Plates may be coated at room temperature.