Fixative

1. Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature., 3. Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm., 4. Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3., 5. When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.

ICT’s Fixative is a formaldehyde solution designed to cross-link intracellular components. It will not interfere with the fluorescence labels used on our fluorescent detection probes like FLICA, Magic Red, and FLISP. Fixative is useful to preserve a cellular staining configuration for 24 hours before cellular analysis. Cells should be stained prior to fixation. This product is included in many of our cell viability assay kits, and can be utilized in a variety of cellular analysis workflows., , Warning: This product is toxic if inhaled, in contact with skin, or if swallowed. Gloves, protective clothing, and eyewear are strongly recommended. When disposing, flush sink with copious amounts of water.