IgM-Reducing Assay Diluent

1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT’s stabilizing blocker products, pipette 50 – 100 µL of Neptune Assay Diluent into each of the Wells of the ELISA plate using a multi-channel pipettor. The Assay Diluent is always added prior to adding the test and control serum/plasma samples to plate Wells., 2. The control and test samples should be diluted to the proper Well concentrations using one of ICT’s three different Sample Diluent products. Using an ICT Sample Diluent to dilute the serum and plasma samples will also serve to help minimize the degree of nonspecific IgG binding to the antigen coated and blocked ELISA plate Wells. The test (sera and plasma) samples, diluted typically to 1:40 or greater in a sample diluent should be incubated in the antigen-coated ELISA plates for 30 – 60 minutes at room temperature or 37°C.

IgM-Reducing Assay Diluent is an ELISA additive specially formulated for use with problematic serum and plasma samples tested in antibody sandwich ELISA formats. This diluent can be added to standard curve/calibrator and serum/plasma sample Wells to help equalize matrix complexity disparity issues., , Large differences in matrix complexity environment between standard curve and sample Wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. Proprietary additives have been included in IgM-Reducing Assay Diluent to reduce IgM-mediated conjugate bridging interference that can otherwise cause substantial background noise., , IgM-Reducing Assay Diluent contains mammalian sera proteins to reduce nonspecific interactions that might occur between the sample matrix proteins and the plate surface. It also contains a proprietary calcium chelating agent designed to inhibit interference from complement and thrombin present in serum and plasma samples. An antimicrobial agent is included in the formulation to allow for bench-top use and storage stability., , Bulk volumes and custom packaging are available upon request.