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Anti-Hu C3b/iC3b Alexa Fluor® 647

Complement component C3 plays a key role in the activation of complement system. In classical complement system pathway the activated C2 and C4 form classical C3-convertase (C4b2b) which cleaves C3 into C3a and C3b. In alternative complement system pathway C3 is cleaved by alternative C3-convertase (C3bBb), composed of C3b (which can be generated also by spontaneous hydrolysis of C3) and the activated form of factor B. C3b activates downstream cascade leading to formation of pores in the plasma membrane of attacked cell. C3b and its proteolytically inactive form iC3b also serve as important opsonins. C3b can generate C3f, as well as the iC3b can be further cleaved into C3c, C3d and C3g. Complement system is regulated by effective inactivation of free C3b by factor H and factor I. C3b attached to the surface of its target is protected from this inactivation. Complement system is also regulated by other proteins, e.g. CD35, CD46, CD55, or CD59. Undesired activation of complement cascade can lead to severe diseases such as PNH, rheumatoid arthritis, macular degeneration and other. Recently it has been demonstrated to take part in complications associated with Covid-19.

Product Specifications

Certification

RUO

Immunogen

Human complement component C3b

Target Antigen

C3b/iC3b

Clone

3E7

Applications

FC (QC tested)

Format

Alexa Fluor® 647

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Li Y, Williams ME, Cousar JB, Pawluczkowycz AW, Lindorfer MA, Taylor RP: Rituximab-CD20 complexes are shaved from Z138 mantle cell lymphoma cells in intravenous and subcutaneous SCID mouse models. J Immunol. 2007 Sep 15;179 (6) :4263-71. , URL: https://pubmed.ncbi.nlm.nih.gov/17785867/, *Kennedy AD, Solga MD, Schuman TA, Chi AW, Lindorfer MA, Sutherland WM, Foley PL, Taylor RP: An anti-C3b (i) mAb enhances complement activation, C3b (i) deposition, and killing of CD20+ cells by rituximab. 2003 Feb 1;101 (3) :1071-9., URL: https://pubmed.ncbi.nlm.nih.gov/12393727/, *Pokrass MJ, Liu MF, Lindorfer MA, Taylor RP: Activation of complement by monoclonal antibodies that target cell-associated β₂-microglobulin: implications for cancer immunotherapy. Mol Immunol. 2013 Dec;56 (4) :549-60., URL: https://pubmed.ncbi.nlm.nih.gov/23911412/, *DiLillo DJ, Pawluczkowycz AW, Peng W, Kennedy AD, Beum PV, Lindorfer MA, Taylor RP: Selective and efficient inhibition of the alternative pathway of complement by a mAb that recognizes C3b/iC3b. Mol Immunol. 2006 Mar;43 (7) :1010-9., URL: https://pubmed.ncbi.nlm.nih.gov/15961157/, *Lindorfer MA, Pawluczkowycz AW, Peek EM, Hickman K, Taylor RP, Parker CJ: A novel approach to preventing the hemolysis of paroxysmal nocturnal hemoglobinuria: both complement-mediated cytolysis and C3 deposition are blocked by a monoclonal antibody specific for the alternative pathway of complement. Blood. 2010 Mar 18;115 (11) :2283-91., URL: https://pubmed.ncbi.nlm.nih.gov/20068220/

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody 3E7 recognizes complement component C3b and iC3b. It does not cross-react with C3d.

Applications Notes

6

Species

Human, Non-human primates

Isotype

Mouse IgG1 kappa

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