PCNA, Biotinylated Antibody

Goat polyclonal antibody against PCNA conjugated to Biotin

Product Specifications

Product Name Alternative

PCNA; proliferating cell nuclear antigen; ATLD2; DNA polymerase delta auxiliary protein; cyclin

Reactivity

Human, Mouse, Porcine, Rat

Target

PCNA, Biotinylated

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NGNIKLSQTSNVD.

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.3-1 μg/ml

Molecular Weight

28.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to NP_002583.1) . See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1μg/ml.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.