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Goat anti-PPP1R12A Antibody

Goat polyclonal antibody to PPP1R12A

Product Specifications

Product Name Alternative

Protein phosphatase 1, regulatory (inhibitor) subunit 12A; MBS; MGC133042; MYPT1; myosin phosphatase, target subunit 1

Reactivity

Human

Target

PPP1R12A

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

REDEYKQKYSRTYD

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.3-0.5 μg/ml, IHC-P: 3.75 μg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Preliminary experiments gave an approx 150kDa band in lysates of cell line MCF7 at 0.3-0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 115kDa according to

Tested Applications

ELISA, FC, IF, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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