PrecipHen® Immunoprecipitation Reagent

Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with chicken antibodies in immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and protein purification applications.

Product Specifications

Background

Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with IP, CHiP, and protein purification applications.

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Goat

Species Reactivity

Chicken

Reactivity

Chicken

Immunogen

n/a

Immunogen Type

n/a

Target

Chicken IgY

Clonality

Polyclonal

Isotype

IgG

Conjugation

Agarose

Applications

Assay Type

Immunoprecipitation Reagent

Assay Protocol

Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Goat anti-chicken IgY antibodies were affinity purified from the sera over a chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS) -activated agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50 mM HEPES buffer (pH 7.6) with 5 mM EDTA (“TE buffer”) . Sodium azide was then added to a final concentration of 0.02% (w/v) .

Format

Agarose beads in buffer, binding capacity 1.0 mg of chicken IgY per ml bed volume 1.0 mg of chicken IgY per ml bed volume

Buffer

Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent)

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, this product should have a shelf life of at least twelve months, provided they remain sterile.

Shelf Life

12 months from receipt of product

Specificity

H&L; does not cross-react with mammalian IgGs.

Pre Treatment

Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Goat anti-chicken IgY antibodies were affinity purified from the sera over a chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50 mM HEPES buffer (pH 7.6) with 5 mM EDTA (“TE buffer”). Sodium azide was then added to a final concentration of 0.02% (w/v).

Quality Control

The capacity of PrecipHen® was determined by measuring the 2.5 mLs of PrecipHen. One milliliter aliquots of chicken IgY (200 µg/mL in TE buffer) were applied to the column and fractions were collected. A similar column of agarose (non-NHS activated) was used for comprison. The concentration of IgY passing through each column was measured, and the total amount of IgY that bound to the column was calculated.