Horseradish Peroxidase Chicken Anti-Rabbit IgG Secondary Antibody

Our Horseradish Peroxidase Chicken Anti-Rabbit IgG secondary antibody from Aves Labs is produced in-house. It detects rabbit IgG and is affinity purified.

Product Specifications

Background

Our Horseradish Peroxidase Chicken Anti-Rabbit IgG secondary antibody from Aves Labs is produced in-house. It detects rabbit IgG and is affinity purified.

Short Description

Our Horseradish Peroxidase Chicken Anti-Rabbit IgG Secondary Antibody from Aves Labs is produced in-house. It detects rabbit igg and is affinity purified.

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Chicken

Species Reactivity

Rabbit

Reactivity

Rabbit

Immunogen

IgG

Immunogen Type

Rabbit

Target

Rabbit IgG

Clonality

Polyclonal

Isotype

IgY

Conjugation

HRP

Applications

IHC, ICC, WB

Assay Type

Secondary Antibody

Assay Protocol

Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains) . Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains) and then conjugated with horseradish peroxidase.

Concentration

1 mg/mL

Format

Affinity Purified

Form

Affinity Purified

Buffer

HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin (0.2%, w/v), thimerosal (0.01%, w/v) (as an anti-microbial agent)

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Specificity

H&L; there is no detectable cross-reactivity with goat, human or mouse IgG.

Pre Treatment

Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG’s were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains) and then conjugated with horseradish peroxidase.

Quality Control

Antibodies were tested for specificity using double-immunodiffusion in agarose gels and ELISA analysis. In the latter, a 1:20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using tetramethylbenzidine as substrate.