Fluoresceinated Chicken Anti-Human IgG Secondary Antibody

Our Fluoresceinated Chicken Anti-Human IgG secondary antibody from Aves Labs is produced in-house. It detects human IgG and is affinity purified.

Product Specifications

Background

Our Fluoresceinated Chicken Anti-Human IgG secondary antibody from Aves Labs is produced in-house. It detects human IgG and is affinity purified.

Short Description

Our Fluoresceinated Chicken Anti-Human IgG Secondary Antibody from Aves Labs is produced in-house. It detects human igg and is affinity purified.

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Chicken

Species Reactivity

Human

Reactivity

Human

Immunogen

IgG

Immunogen Type

Human

Target

Human IgG

Clonality

Polyclonal

Isotype

IgY

Conjugation

FITC

Applications

IHC, ICC, WB

Assay Type

Secondary Antibody

Assay Protocol

Chickens were immunized with purified, non-denatured human IgG (containing both heavy and light chains) . Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-human IgG’s were affinity-purified over a column conjugated with purified human IgG (containing both heavy and light chains) and then conjugated with fluorescein isothiocyanate.

Concentration

1 mg/mL

Format

Affinity Purified

Form

Affinity Purified

Buffer

Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent)

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Specificity

H&L; there is no detectable cross-reactivity with goat, mouse or rabbit IgG.

Pre Treatment

Chickens were immunized with purified, non-denatured human IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-human IgG’s were affinity-purified over a column conjugated with purified human IgG (containing both heavy and light chains) and then conjugated with fluorescein isothiocyanate.

Quality Control

Antibodies were tested for specificity using double-immunodiffusion in agarose and for non-specific binding using cryostat sections of unfixed embryonic chick tissues.