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Chicken Anti-Goat IgG Secondary Antibody

Our Chicken Anti-Goat IgG secondary antibody from Aves Labs is produced in-house. It detects goat IgG and is affinity purified.

Product Specifications

Background

Our Chicken Anti-Goat IgG secondary antibody from Aves Labs is produced in-house. It detects goat IgG and is affinity purified.

Short Description

Our Chicken Anti-Goat IgG Secondary Antibody from Aves Labs is produced in-house. It detects goat igg and is affinity purified.

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Chicken

Species Reactivity

Goat

Reactivity

Goat

Immunogen

IgG

Immunogen Type

Goat

Target

Goat IgG

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Assay Type

Secondary Antibody

Assay Protocol

Chickens were immunized with purified, non-denatured goat IgG (containing both heavy and light chains) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG’s were affinity-purified over a column conjugated with purified goat IgG (whole protein) .

Concentration

1 mg/mL

Format

Affinity Purified

Form

Affinity Purified

Buffer

Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent)

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Specificity

H&L; there is no detectable cross-reactivity with rabbit, human or mouse IgG.

Pre Treatment

Chickens were immunized with purified, non-denatured goat IgG (containing both heavy and light chains) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG’s were affinity-purified over a column conjugated with purified goat IgG (whole protein).

Quality Control

Antibodies were tested for specificity using double-immunodiffusion assays in agarose.

Brid

AB_2313522

Physical Properties

Liquid

Isotype

IgY

Curated Selection

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