Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody

Our Fluoresceinated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.

Product Specifications

Background

Our Fluoresceinated Goat Anti-Chicken IgY secondary antibody from Aves Labs is produced in-house. It detects chicken IgY and is affinity purified.

Short Description

Our Fluoresceinated Goat Anti-Chicken IgY Secondary Antibody from Aves Labs is produced in-house. It detects chicken igy and is affinity purified.

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Goat

Species Reactivity

Chicken

Reactivity

Chicken

Immunogen

IgY

Immunogen Type

Chicken

Target

Chicken IgY

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Applications

IHC, ICC, WB

Assay Type

Secondary Antibody

Assay Protocol

Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to fluorescein isothiocyanate.

Concentration

1 mg/mL

Format

Affinity Purified

Form

Affinity Purified

Buffer

Phosphate-buffered saline (PBS) (pH 7.2), bovine serum albumin (0.2%, w/v), sodium azide (0.02%, w/v) (as an anti-microbial agent)

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:500-1:1000

Specificity

H&L; does not cross-react with mammalian IgGs.

Pre Treatment

ICC Dilution

1:500-1:1000

Quality Control

Antibodies were analyzed using immunoelectrophoresis and immunocytochemistry. In this latter test, abundant fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1:10 dilution in PBS), incubation with chicken anti-vimentin antibodies (1:1000 dilution in PBS) and then incubation with GGHL-30F-2 (1:200 dilution in PBS) .