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Anti-Vimentin Protein Antibody

Three different antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_035831) and human (NP_003371) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

We produce our Anti-Vimentin chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

Synonyms

Vimentin

Gene Name

VIM

UniProt

P08670

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Immunogen

Human, Mouse, Rat

Immunogen Type

Synthetic peptides

Target

Vimentin

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Assay Type

Primary Antibody

Concentration

300 µg/mL

Format

Liquid

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

52 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.gentaur.com/products/588/688a1e1a118489d09abbf63f/datasheet/vim.pdf

IHC Dilution

1:1000-1:2000

WB Dilution

1:2000-1:5000

Pre Treatment

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the vimentin gene product, but are shared between the human (NP_003371, NCBI) and mouse (NP_035831, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter sterilized.

Target Description

Human Vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, Vimentin is expressed by migrating neural crest cells forming the PNS and by neural stem cells of the CNS. In some forms of glioblastoma, Vimenin is re-expressed by the tumor cells and serves as a general indicator of their undifferentiated state.

ICC Dilution

1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313565

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