Anti-V5 Antibody

Our Anti-V5 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects V5, and is affinity purified. It is great for use in ICC, WB.

Product Specifications

Background

The V5 tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.

Short Description

Our Anti-V5 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a V5, and is affinity purified. It is great for use in ICC, WB.

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Host

Chicken

Immunogen

Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein

Immunogen Type

Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein

Target

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

ICC, WB

Field of Research

Anti-Tag Antibodies

Assay Type

Primary Antibody

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Concentration

0.2 mg/mL

Format

Affinity purified

Form

Affinity purified

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

WB Dilution

1:500-1:1000

Pre Treatment

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Target Description

ICC Dilution

1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on a V5 overexpression cell lysate.