Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-Tyrosine Hydroxylase (TYH) Antibody

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P24529) and human (P07101) gene products. Antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

We produce our Anti-Tyrosine Hydroxylase (TH) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

Synonyms

Tyrosine 3-monooxygenase, EC 1.14.16.2, Tyrosine 3-hydroxylase, TH, TYH

Gene Name

TH TYH

UniProt

P07101

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Immunogen

Human, Mouse, Rat

Immunogen Type

Synthetic peptides

Target

Tyrosine Hydroxylase (TH)

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Assay Type

Primary Antibody

Concentration

200 µg/mL

Format

Liquid

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

60 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.gentaur.com/products/588/688a1e1a118489d09abbf666/datasheet/tyh-0020.pdf

IHC Dilution

1:1000-1:2000

WB Dilution

1:1000-1:2000

Pre Treatment

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101, NCBI) and mouse (P24529, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized.

Target Description

Human TYH (EC 1.14.16.2) is a 58,523 dalton protein (528 amino acids) responsible for the enzymatic conversion of L-tyrosine to L-DOPA (dihydroxyphenylalanine). This enzyme is expressed in all catecholaminergic neurons of the CNS and PNS. In the CNS, TYH-positive neurons can be found within the substantia nigra, ventral tegmental area, locus ceruleus, and hypothalamus. In the PNS, TYH-positive neurons can be found within the sympathetic chain, pre-vertebral ganglia and the adrenal medulla.

ICC Dilution

1:1000-1:2000

Quality Control

Both of the antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_10013440

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Phospho-CdGAP (Thr789) Blocking Peptide
MBS9619215-01 1 mg

Phospho-CdGAP (Thr789) Blocking Peptide

Ask
View Details
Phospho-CdGAP (Thr789) Blocking Peptide
MBS9619215-02 5x 1 mg

Phospho-CdGAP (Thr789) Blocking Peptide

Ask
View Details
Rat NEGR1 siRNA
abx925700-01 15 nmol

Rat NEGR1 siRNA

Ask
View Details
Rat NEGR1 siRNA
abx925700-02 30 nmol

Rat NEGR1 siRNA

Ask
View Details
IRGM siRNA (Rat)
MBS8202322-01 15 nmol

IRGM siRNA (Rat)

Ask
View Details
IRGM siRNA (Rat)
MBS8202322-02 30 nmol

IRGM siRNA (Rat)

Ask
View Details