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Anti-Beta-Tubulin 3 (TUJ) Antibody

Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.

Synonyms

Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

TUBB3 TUBB4

UniProt

Q13509

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic peptides

Immunogen Type

Human, Mouse, Rat

Target

Beta-Tubulin 3

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Field of Research

Cell Markers Neuronal Markers

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Concentration

300 µg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

50 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/TUJ_datasheet.pdf

IHC Dilution

1:2000-1:5000

WB Dilution

1:10000-1:20000

Pre Treatment

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Target Description

Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.

ICC Dilution

1:2000-1:5000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313564

Uniprot Accession Number

Q13509

Physical Properties

Liquid

Isotype

IgY

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