Anti-TPH2 Antibody

Our Anti-THP2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat TPH2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

Product Specifications

Background

Tryptophan hydroxylase 2 (TPH2) is an enzyme involved in the biosynthesis of serotonin, a neurotransmitter that plays a crucial role in regulating mood, appetite, and sleep. TPH2 specifically catalyzes the conversion of tryptophan to 5-hydroxytryptophan (5-HTP), a precursor to serotonin. Unlike its counterpart TPH1, which is primarily found in the periphery, TPH2 is predominantly expressed in the central nervous system, particularly in the raphe nuclei of the brainstem. The genetic regulation and expression of TPH2 have been extensively studied in the context of various psychiatric disorders, including depression and anxiety. Research on TPH2 aids in the development of targeted therapeutic interventions focusing on modulating serotonin levels in the brain. Recent studies have shown TPH2 acting in a sex-dependent manner for body processes such as energy balance (Liu et al. Endocrinology, 2021) and anxiety behaviors (Li et al. Neuropharmacology, 2022) .

Short Description

Our Anti-THP2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat TPH2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

Synonyms

Tryptophan Hydroxylase 2, NTPH, Tryptophan 5-Monooxygenase 2, Tryptophan 5-Hydroxylase 2, ADHD7

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

TPH2

UniProt

Q8IWU9

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Two synthetic peptides located near the C-terminus of TPH2 (Uniprot - Q8IWU9)

Immunogen Type

Human, Mouse, Rat

Target

TPH2

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

ICC, IHC, WB

Field of Research

Cell Markers Neuronal MarkersSerotonergic Neuronal Markers

Assay Type

Primary Antibody

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Concentration

200 µg/mL

Dilution

WB Brain : 1:100-1:1000

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

56 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:100-1:500

Specificity

No cross-reactivity reported

Pre Treatment

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Target Description

ICC Dilution

1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.