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Anti-Tau Antibody

Antipeptide antibodies were generated in chickens against two sequences shared between the mouse (P10637.3) and human (P10636.5) gene products. Neither of these sequences contain phosphorylated serine or threonine residues, so these Tau antibodies recognize both the euphosphorylated and hyperphosphorylated forms of Tau. Antibodies were affinity-purified from IgY fractions prepared from yolks collected from hyperimmunized hens. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

Human Tau protein (i.e., microtubule-associated protein tau isoform 1) is a 78,902 dalton protein (758 amino acids) expressed in cortical neurons of the CNS. In normal individuals, tau contains moderate degrees of phosphorylation on various serine and threonine residues scattered throughout the N-terminal half of this protein. However in various neurodegenerative diseases, such as Alzheimer's disease, Tau becomes hyperphosphorylated on these residues, leading to the pathological formation of intracellular "neurofibrillary tangles." Such tangles are hallmarks of Alzheimer's disease, as well as various other neurodegenerative diseases, collectively known as the "tauopathies."

Synonyms

Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

MAPT MAPTL MTBT1 TAU

UniProt

P10636

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Synthetic peptides

Immunogen Type

Human, Mouse, Rat

Target

Tau

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC

Field of Research

Cell Markers Neuronal Markers Axonal Markers Neurodegenerative Disease Alzheimer's Disease

Assay Type

Primary Antibody

Assay Protocol

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different and nonphosphorylated regions of the Tau gene product, but are shared between the human (NP_05819, NCBI) and mouse (P01637, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Concentration

200 µg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

55-62 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:5000-1:10000

Pre Treatment

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different and nonphosphorylated regions of the Tau gene product, but are shared between the human (NP_05819, NCBI) and mouse (P01637, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Target Description

Human Tau protein (i.e., microtubule-associated protein tau isoform 1) is a 78,902 dalton protein (758 amino acids) expressed in cortical neurons of the CNS. In normal individuals, tau contains moderate degrees of phosphorylation on various serine and threonine residues scattered throughout the N-terminal half of this protein. However in various neurodegenerative diseases, such as Alzheimer's disease, Tau becomes hyperphosphorylated on these residues, leading to the pathological formation of intracellular "neurofibrillary tangles." Such tangles are hallmarks of Alzheimer's disease, as well as various other neurodegenerative diseases, collectively known as the "tauopathies."

ICC Dilution

1:5000-1:10000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313563

Uniprot Accession Number

P10636

Physical Properties

Liquid

Isotype

IgY

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