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Anti-Peripherin Antibody

Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Background

We produce our Anti-Perpherin chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is IgY fraction.

Synonyms

Peripherin, Neurofilament 4

Gene Name

PRPH NEF4 PRPH1

UniProt

P41219

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Immunogen

Rat

Immunogen Type

Recombinant rat peripherin expressed in Escherichia coli

Target

Perpherin

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Assay Type

Primary Antibody

Concentration

2 mg/mL

Format

Liquid

Form

IgY Fraction

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Molecular Weight

60 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.gentaur.com/products/588/688a1e1a118489d09abbf63a/datasheet/per.pdf

IHC Dilution

1:2000-1:5000

WB Dilution

1:2000-1:5000

Pre Treatment

Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.

Target Description

Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.

ICC Dilution

1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Brid

AB_2313558

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