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Anti-Olig2

Our Anti-Olig2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Olig2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

Product Specifications

Background

OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008) . OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.

Short Description

Our Anti-Olig2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat Olig2, and is affinity-purified igy. It is great for use in IHC, ICC, WB.

Synonyms

BHLHB1, BHLHE19, PRKCBP2, RACK17

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

OLIG2

UniProt

Q13516

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

N-terminal half of human OLIG2 was used to immunize chickens to avoid cross-reactivity with OLIG3.

Immunogen Type

Human

Target

Olig2

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

IHC, ICC, WB

Field of Research

Cell Markers Glial and Schwann Cell MarkersNeurogenesis and Development Transcription Regulation

Assay Type

Primary Antibody

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Concentration

0.2 mg/mL

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

35 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

Product Datasheet

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/OLIG2__datasheet.pdf?v=1666123088

IHC Dilution

1:1000

WB Dilution

1:1000

Pre Treatment

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Target Description

OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008). OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.

ICC Dilution

1:1000

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing Olig2.

Brid

AB_2924438

Uniprot Accession Number

Q13516

Physical Properties

Liquid

Isotype

IgY

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