Anti-NDRG2 Antibody

Our Anti-NDRG2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat NDRG2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

Product Specifications

Background

NDRG2, or N-myc downstream-regulated gene 2, is a downstream target of the N-myc proto-oncogene. NDRG2 was initially identified for its role in cell differentiation and response to cellular stress. Multiple studies have determined that NDRG2 mRNA is downregulated or undetectable in several human cancers, and over-expression of NDRG2 inhibited the proliferation of cancer cells. Commonly regarded as a tumor suppressor, NDRG2 also plays a crucial role in regulating cell proliferation, apoptosis, and metastasis. In neuroscience, studies of astrocytes in the central nervous system have implicated NDRG2 in the pathogenesis of many neurological diseases not limited to tumors; including stroke, neurodegenerative diseases, and psychiatric disorders. Its involvement in maintaining the integrity of the blood-brain barrier adds to its significance in neurobiology (Takarada-Iemata et al. Glia, 2018) . Drug therapies to modulate the expression of NDGR2 may be able to help patients suffering from disease states ranging from multiple cancer types to Alzheimer’s, Parkinson’s, and depression.

Short Description

Our Anti-NDRG2 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat NDRG2, and is affinity-purified IgY. It is great for use in IHC, ICC, WB.

Synonyms

SYLD, KIAA1248, N-Myc Downstream-Regulated Gene 2 Protein, N-Myc Downstream Regulator 2, NDR1-Related Protein NDR2, Cytoplasmic Protein Ndr1, Syld709613 Protein

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

NDRG2

UniProt

Q9UN36

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Recombinant protein encompassing the N-terminal ~150 amino acids of human NDRG2

Immunogen Type

Human, Mouse, Rat

Target

NDRG2

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

ICC, IHC, WB

Field of Research

Cell Markers Glial and Schwann Cell Markers Astrocyte Markers

Assay Type

Primary Antibody

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Concentration

200 µg/mL

Dilution

WB Brain : 1:500-1:1000

Format

Affinity-Purified IgY

Form

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

41 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Shelf Life

12 months from receipt of product

IHC Dilution

1:100-1:500

Specificity

No cross-reactivity reported

Pre Treatment

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

Target Description

NDRG2, or N-myc downstream-regulated gene 2, is a downstream target of the N-myc proto-oncogene. NDRG2 was initially identified for its role in cell differentiation and response to cellular stress. Multiple studies have determined that NDRG2 mRNA is downregulated or undetectable in several human cancers, and over-expression of NDRG2 inhibited the proliferation of cancer cells. Commonly regarded as a tumor suppressor, NDRG2 also plays a crucial role in regulating cell proliferation, apoptosis, and metastasis. In neuroscience, studies of astrocytes in the central nervous system have implicated NDRG2 in the pathogenesis of many neurological diseases not limited to tumors; including stroke, neurodegenerative diseases, and psychiatric disorders. Its involvement in maintaining the integrity of the blood-brain barrier adds to its significance in neurobiology (Takarada-Iemata et al. Glia, 2018). Drug therapies to modulate the expression of NDGR2 may be able to help patients suffering from disease states ranging from multiple cancer types to Alzheimer’s, Parkinson’s, and depression.

ICC Dilution

1:100-1:500

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.